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Agarose gel electrophoresis of biotinylated double and single stranded DNA produced through amplification of specific regions in M13 ssDNA using biotinylated and phosphorylated primers. Singe stranded DNA (ssDNA) was produced after treating the PCR produced dsDNA with lambda exonuclease.

Journal: Biomaterials Science

Article Title: Controlling cellular packing and hypoxia in 3D tumor spheroids via DNA interactions †

doi: 10.1039/d4bm00688g

Figure Lengend Snippet: Agarose gel electrophoresis of biotinylated double and single stranded DNA produced through amplification of specific regions in M13 ssDNA using biotinylated and phosphorylated primers. Singe stranded DNA (ssDNA) was produced after treating the PCR produced dsDNA with lambda exonuclease.

Article Snippet: The phosphorylated strand digestion was done using the Lambda Exonuclease from NEB.

Techniques: Agarose Gel Electrophoresis, Produced, Amplification